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Foi avaliado a morfologia das células crescendo sobre amostras de hidrogéis e sobre lamínulas de vidro, bem como a presença e viabilidade das células presentes nos sobrenadantes (método de exclusão com azul Tripan). Polipropileno e adevivo de silicone foram utilizados como controles negativo e positivo respectivamente. Resumo Hidrogéis de poli(2-hidróxi etil metacrilato) reticulado e blendas do tipo redes semi interpenetrantes (sIPN) de poliHEMA com acetato de celulose e poliHEMA com poli(metacrilato de metila-co-ácido acrílico), desenvol-vidas objetivando aplicação no reparo de defeitos da carti-lagem articular foram submetidas a testes de citotoxici-dade in vitro usando células VERO.

Citotoxicity evaluation for polyhema hydrogels: an in vitro study. Hidrogéis de polihema: um estudo in vitro. At higher concentrations (5 and 10 wt %), and higher molecular weights (920, 1400, and 2250 g mol⁻¹), the PE-b-PEG copolymers leached-out resulting in protein adsorption comparable to that of neat PP. The PP blends compounded with PE-b-PEG copolymer having the lowest molecular weight (875 g mol⁻¹), at the lowest concentration (1 wt %), gave the lowest bovine serum protein adsorption (30% less) compared to that of neat PP. Enrichment of PEG chains at the surface of the blends increased with increasing PE-b-PEG copolymer concentration and molecular weight. Phase separation of the PE-b-PEG copolymer and its migration to the surface of the PP blend, was confirmed by attenuated total reflectance–Fourier transform infrared, X-ray photoelectron spectroscopy, and static water contact angle measurements. Protein-resistant polyethylene-block-poly(ethylene glycol) (PE-b-PEG) copolymers of different molecular weights at various concentrations were compounded by melt blending with polypropylene (PP) polymers in order to enhance their antifouling properties.

Carti de cultura generala pdf merge download